vector network analyzer e5061b Search Results


vector network analyzer  (Agilent technologies)
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Agilent technologies vector network analyzer
Vector Network Analyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e5061 vector network analyzer  (Keysight Technologies)
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Keysight Technologies e5061 vector network analyzer
E5061 Vector Network Analyzer, supplied by Keysight Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e5061b ena vector network analyzer  (Keysight Technologies)
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Keysight Technologies e5061b ena vector network analyzer
E5061b Ena Vector Network Analyzer, supplied by Keysight Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vector network analyzer e5061b, santa clara, ca  (Agilent technologies)
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Agilent technologies vector network analyzer e5061b, santa clara, ca
Vector Network Analyzer E5061b, Santa Clara, Ca, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vector network analyzer e5061b, santa clara, ca - by Bioz Stars, 2026-04
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pgl4.54[luc2/tk] vector  (Promega)
90
Promega pgl4.54[luc2/tk] vector
Nuclear SLC14A1 plays a tumor suppressor role through recruitment of HDAC1 to transrepress the HK2 gene. XTT, cell proliferation, and Boyden chamber assays along with immunocytofluorescence and confocal microscopy were performed in vitro. Either SLC14A1 or SLC14A1-NLS (nuclear form) overexpression suppressed cell viability, proliferation, migration and invasion (A) and induced mitochondrial fusion (B) in UMUC3 cells compared to the mock . (C, D) Either SLC14A1 or SLC14A1-NLS overexpression downregulated SLC2A1, HK2, PKM, LDHA/C, pMTOR(S2448), and pRPS6(S235) and upregulated PDHA1 and MFN2 protein levels in UMUC3 cells compared to the mock . (E) Subcutaneous injection of SLC14A1-NLS -overexpressing UMUC3 cells into NOD/SCID mice ( n = 8) suppressed tumor growth compared to the mock . (F, G) Immunohistochemistry showed that SLC14A1, PDHA1 and MFN2 protein levels were notably upregulated, whereas pMTOR(S2448), pRPS6(S235), Ki-67, SLC2A1, HK2, PKM and LDHA/C protein levels were markedly downregulated in xenografts from SLC14A1-NLS -overexpressing UMUC3 cells, similar to those of xenografts from SLC14A1 -overexpressing UMUC3 cells. (H) Quantitative RT-PCR showed that overexpression of the SLC14A1-NLS gene downregulated the HK2 mRNA level. (I) Cotransfection of the pKM2L-PhHKII (region #1) and <t>pGL4.54[luc2/TK]</t> plasmids into stable SLC14A1-NLS -overexpressing UMUC3 cells along with the Dual-Glo® Luciferase Reporter Assay displayed that HK2 promoter activity was downregulated compared to the mock . (J) Coimmunoprecipitation by probing anti-SLC14A1 antibody (αSLC14A1) and immunoblot with anti-SIN3A or anti-HDAC1 antibody confirmed that SLC14A1 interacts with SIN3A and HDAC1 in both RTCC1 and BFTC905 cells. (K) The TRANSFAC® database predicted three putative HDAC1-responsive elements in the promoter region of the HK2 gene, and a quantitative ChIP assay validated that SCL14A1-NLS overexpression increased the SCL14A1-NLS binding folds at site 1, site 2 and site 3. All experiments were performed in triplicate, and the results are expressed as the mean ± SD. For the immunocytofluorescence, immunoblot and immunohistochemistry assays, representative images are shown. Actin, beta (ACTB) served as a loading control for immunoblot analysis. Statistical significance: * P < 0.05.
Pgl4.54[Luc2/Tk] Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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firefly luciferase vector pgl4.54 (tk-firefly  (Promega)
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Promega firefly luciferase vector pgl4.54 (tk-firefly
Nuclear SLC14A1 plays a tumor suppressor role through recruitment of HDAC1 to transrepress the HK2 gene. XTT, cell proliferation, and Boyden chamber assays along with immunocytofluorescence and confocal microscopy were performed in vitro. Either SLC14A1 or SLC14A1-NLS (nuclear form) overexpression suppressed cell viability, proliferation, migration and invasion (A) and induced mitochondrial fusion (B) in UMUC3 cells compared to the mock . (C, D) Either SLC14A1 or SLC14A1-NLS overexpression downregulated SLC2A1, HK2, PKM, LDHA/C, pMTOR(S2448), and pRPS6(S235) and upregulated PDHA1 and MFN2 protein levels in UMUC3 cells compared to the mock . (E) Subcutaneous injection of SLC14A1-NLS -overexpressing UMUC3 cells into NOD/SCID mice ( n = 8) suppressed tumor growth compared to the mock . (F, G) Immunohistochemistry showed that SLC14A1, PDHA1 and MFN2 protein levels were notably upregulated, whereas pMTOR(S2448), pRPS6(S235), Ki-67, SLC2A1, HK2, PKM and LDHA/C protein levels were markedly downregulated in xenografts from SLC14A1-NLS -overexpressing UMUC3 cells, similar to those of xenografts from SLC14A1 -overexpressing UMUC3 cells. (H) Quantitative RT-PCR showed that overexpression of the SLC14A1-NLS gene downregulated the HK2 mRNA level. (I) Cotransfection of the pKM2L-PhHKII (region #1) and <t>pGL4.54[luc2/TK]</t> plasmids into stable SLC14A1-NLS -overexpressing UMUC3 cells along with the Dual-Glo® Luciferase Reporter Assay displayed that HK2 promoter activity was downregulated compared to the mock . (J) Coimmunoprecipitation by probing anti-SLC14A1 antibody (αSLC14A1) and immunoblot with anti-SIN3A or anti-HDAC1 antibody confirmed that SLC14A1 interacts with SIN3A and HDAC1 in both RTCC1 and BFTC905 cells. (K) The TRANSFAC® database predicted three putative HDAC1-responsive elements in the promoter region of the HK2 gene, and a quantitative ChIP assay validated that SCL14A1-NLS overexpression increased the SCL14A1-NLS binding folds at site 1, site 2 and site 3. All experiments were performed in triplicate, and the results are expressed as the mean ± SD. For the immunocytofluorescence, immunoblot and immunohistochemistry assays, representative images are shown. Actin, beta (ACTB) served as a loading control for immunoblot analysis. Statistical significance: * P < 0.05.
Firefly Luciferase Vector Pgl4.54 (Tk Firefly, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e5061 vector network analyzer  (Agilent technologies)
90
Agilent technologies e5061 vector network analyzer
Nuclear SLC14A1 plays a tumor suppressor role through recruitment of HDAC1 to transrepress the HK2 gene. XTT, cell proliferation, and Boyden chamber assays along with immunocytofluorescence and confocal microscopy were performed in vitro. Either SLC14A1 or SLC14A1-NLS (nuclear form) overexpression suppressed cell viability, proliferation, migration and invasion (A) and induced mitochondrial fusion (B) in UMUC3 cells compared to the mock . (C, D) Either SLC14A1 or SLC14A1-NLS overexpression downregulated SLC2A1, HK2, PKM, LDHA/C, pMTOR(S2448), and pRPS6(S235) and upregulated PDHA1 and MFN2 protein levels in UMUC3 cells compared to the mock . (E) Subcutaneous injection of SLC14A1-NLS -overexpressing UMUC3 cells into NOD/SCID mice ( n = 8) suppressed tumor growth compared to the mock . (F, G) Immunohistochemistry showed that SLC14A1, PDHA1 and MFN2 protein levels were notably upregulated, whereas pMTOR(S2448), pRPS6(S235), Ki-67, SLC2A1, HK2, PKM and LDHA/C protein levels were markedly downregulated in xenografts from SLC14A1-NLS -overexpressing UMUC3 cells, similar to those of xenografts from SLC14A1 -overexpressing UMUC3 cells. (H) Quantitative RT-PCR showed that overexpression of the SLC14A1-NLS gene downregulated the HK2 mRNA level. (I) Cotransfection of the pKM2L-PhHKII (region #1) and <t>pGL4.54[luc2/TK]</t> plasmids into stable SLC14A1-NLS -overexpressing UMUC3 cells along with the Dual-Glo® Luciferase Reporter Assay displayed that HK2 promoter activity was downregulated compared to the mock . (J) Coimmunoprecipitation by probing anti-SLC14A1 antibody (αSLC14A1) and immunoblot with anti-SIN3A or anti-HDAC1 antibody confirmed that SLC14A1 interacts with SIN3A and HDAC1 in both RTCC1 and BFTC905 cells. (K) The TRANSFAC® database predicted three putative HDAC1-responsive elements in the promoter region of the HK2 gene, and a quantitative ChIP assay validated that SCL14A1-NLS overexpression increased the SCL14A1-NLS binding folds at site 1, site 2 and site 3. All experiments were performed in triplicate, and the results are expressed as the mean ± SD. For the immunocytofluorescence, immunoblot and immunohistochemistry assays, representative images are shown. Actin, beta (ACTB) served as a loading control for immunoblot analysis. Statistical significance: * P < 0.05.
E5061 Vector Network Analyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e5061 vector network analyzer/product/Agilent technologies
Average 90 stars, based on 1 article reviews
e5061 vector network analyzer - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


Nuclear SLC14A1 plays a tumor suppressor role through recruitment of HDAC1 to transrepress the HK2 gene. XTT, cell proliferation, and Boyden chamber assays along with immunocytofluorescence and confocal microscopy were performed in vitro. Either SLC14A1 or SLC14A1-NLS (nuclear form) overexpression suppressed cell viability, proliferation, migration and invasion (A) and induced mitochondrial fusion (B) in UMUC3 cells compared to the mock . (C, D) Either SLC14A1 or SLC14A1-NLS overexpression downregulated SLC2A1, HK2, PKM, LDHA/C, pMTOR(S2448), and pRPS6(S235) and upregulated PDHA1 and MFN2 protein levels in UMUC3 cells compared to the mock . (E) Subcutaneous injection of SLC14A1-NLS -overexpressing UMUC3 cells into NOD/SCID mice ( n = 8) suppressed tumor growth compared to the mock . (F, G) Immunohistochemistry showed that SLC14A1, PDHA1 and MFN2 protein levels were notably upregulated, whereas pMTOR(S2448), pRPS6(S235), Ki-67, SLC2A1, HK2, PKM and LDHA/C protein levels were markedly downregulated in xenografts from SLC14A1-NLS -overexpressing UMUC3 cells, similar to those of xenografts from SLC14A1 -overexpressing UMUC3 cells. (H) Quantitative RT-PCR showed that overexpression of the SLC14A1-NLS gene downregulated the HK2 mRNA level. (I) Cotransfection of the pKM2L-PhHKII (region #1) and pGL4.54[luc2/TK] plasmids into stable SLC14A1-NLS -overexpressing UMUC3 cells along with the Dual-Glo® Luciferase Reporter Assay displayed that HK2 promoter activity was downregulated compared to the mock . (J) Coimmunoprecipitation by probing anti-SLC14A1 antibody (αSLC14A1) and immunoblot with anti-SIN3A or anti-HDAC1 antibody confirmed that SLC14A1 interacts with SIN3A and HDAC1 in both RTCC1 and BFTC905 cells. (K) The TRANSFAC® database predicted three putative HDAC1-responsive elements in the promoter region of the HK2 gene, and a quantitative ChIP assay validated that SCL14A1-NLS overexpression increased the SCL14A1-NLS binding folds at site 1, site 2 and site 3. All experiments were performed in triplicate, and the results are expressed as the mean ± SD. For the immunocytofluorescence, immunoblot and immunohistochemistry assays, representative images are shown. Actin, beta (ACTB) served as a loading control for immunoblot analysis. Statistical significance: * P < 0.05.

Journal: Theranostics

Article Title: SLC14A1 prevents oncometabolite accumulation and recruits HDAC1 to transrepress oncometabolite genes in urothelial carcinoma

doi: 10.7150/thno.51655

Figure Lengend Snippet: Nuclear SLC14A1 plays a tumor suppressor role through recruitment of HDAC1 to transrepress the HK2 gene. XTT, cell proliferation, and Boyden chamber assays along with immunocytofluorescence and confocal microscopy were performed in vitro. Either SLC14A1 or SLC14A1-NLS (nuclear form) overexpression suppressed cell viability, proliferation, migration and invasion (A) and induced mitochondrial fusion (B) in UMUC3 cells compared to the mock . (C, D) Either SLC14A1 or SLC14A1-NLS overexpression downregulated SLC2A1, HK2, PKM, LDHA/C, pMTOR(S2448), and pRPS6(S235) and upregulated PDHA1 and MFN2 protein levels in UMUC3 cells compared to the mock . (E) Subcutaneous injection of SLC14A1-NLS -overexpressing UMUC3 cells into NOD/SCID mice ( n = 8) suppressed tumor growth compared to the mock . (F, G) Immunohistochemistry showed that SLC14A1, PDHA1 and MFN2 protein levels were notably upregulated, whereas pMTOR(S2448), pRPS6(S235), Ki-67, SLC2A1, HK2, PKM and LDHA/C protein levels were markedly downregulated in xenografts from SLC14A1-NLS -overexpressing UMUC3 cells, similar to those of xenografts from SLC14A1 -overexpressing UMUC3 cells. (H) Quantitative RT-PCR showed that overexpression of the SLC14A1-NLS gene downregulated the HK2 mRNA level. (I) Cotransfection of the pKM2L-PhHKII (region #1) and pGL4.54[luc2/TK] plasmids into stable SLC14A1-NLS -overexpressing UMUC3 cells along with the Dual-Glo® Luciferase Reporter Assay displayed that HK2 promoter activity was downregulated compared to the mock . (J) Coimmunoprecipitation by probing anti-SLC14A1 antibody (αSLC14A1) and immunoblot with anti-SIN3A or anti-HDAC1 antibody confirmed that SLC14A1 interacts with SIN3A and HDAC1 in both RTCC1 and BFTC905 cells. (K) The TRANSFAC® database predicted three putative HDAC1-responsive elements in the promoter region of the HK2 gene, and a quantitative ChIP assay validated that SCL14A1-NLS overexpression increased the SCL14A1-NLS binding folds at site 1, site 2 and site 3. All experiments were performed in triplicate, and the results are expressed as the mean ± SD. For the immunocytofluorescence, immunoblot and immunohistochemistry assays, representative images are shown. Actin, beta (ACTB) served as a loading control for immunoblot analysis. Statistical significance: * P < 0.05.

Article Snippet: pKM2L-phHKII (region #1, RDB05882, RIKEN, Renilla luciferase) and pGL4.54[luc2/TK] Vector (E5061, Promega, USA) were cotransfected into the mock - and SLC14A1-NLS -overexpressing J82 cells in a 96-well white plate by using PolyJet™ In Vitro DNA Transfection Reagent (Signagen®, USA).

Techniques: Confocal Microscopy, In Vitro, Over Expression, Migration, Injection, Immunohistochemistry, Quantitative RT-PCR, Cotransfection, Luciferase, Reporter Assay, Activity Assay, Western Blot, Binding Assay